Afsaneh Farjami 1, Sina Jalilzadeh 2, Mohammadreza Siahi-Shadbad 3, Farzaneh Lotfipour 4
Abstract
Biofilms are considered a significant reason for the failure of disinfection strategies in industrial water systems due to their resistance to antimicrobial agents.
This study is designed to investigate the anti-biofilm activity of hydrogen peroxide (H2O2) at combinations of temperatures and contact times. For this purpose, an in vitro microtiter plate (MTP)-based model system was used for biofilm formation using Escherichia coli (E. coli) strain FL-Tbz isolated from the water system of a pharmaceutical plant.
To investigate the anti-biofilm activity of H2O2, it was added at different concentrations (2-7% v/v) to biofilms and incubated at different temperatures (20-60 °C) for 10-40 min to find effective conditions to eradicate biofilms. Maximum biofilms were formed when bacterial suspensions were incubated at 37 °C for 96 h.
The rate of biofilm formation using an environmental isolate was higher than that of standard strain.
H2O2 at concentrations of ≥6.25% (v/v) at temperatures of ≥40 °C incubated for ≥25 min significantly eradicated the biofilms.
Journal of Water & Health
Research Articel
The anti-biofilm activity of hydrogen peroxide against Escherichia coli strain FL-Tbz
